Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
overexpression in Escherichia coli | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00058 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.011 | - |
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine | pH 8.0, 37°C, wild type enzyme | Escherichia coli | |
0.03 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 37°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.12 | - |
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine | pH 8.0, 37°C, wild type enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26630 | - |
MALDI TOF mass spectrometry | Sulfurisphaera tokodaii |
35310 | - |
MALDI TOF mass spectrometry | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Sulfurisphaera tokodaii | F9VNG7 | - |
- |
Sulfurisphaera tokodaii 7 | F9VNG7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + [Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine | biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. Biotin carboxyl carrier protein becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase | Escherichia coli | AMP + diphosphate + [Escherichia coli biotin-carboxyl-carrier-protein mutant M136S]-biotinyl-L-lysine | - |
? | |
ATP + biotin + [Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine | biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. Biotin carboxyl carrier protein becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase | Escherichia coli | AMP + diphosphate + [Escherichia coli biotin-carboxyl-carrier-protein]-biotinyl-L-lysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? | |
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
STK_15250 | locus name | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.0097 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 37°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.165 | - |
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine | pH 8.0, 37°C, wild type enzyme | Escherichia coli | |
0.58 | - |
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine | pH 8.0, 37°C, wild type enzyme | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 37°C, wild type enzyme | Sulfurisphaera tokodaii | |
0.49 | - |
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine | pH 8.0, 37°C, wild type enzyme | Escherichia coli | |
6.9 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
15 | - |
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine | pH 8.0, 37°C, wild type enzyme | Escherichia coli |