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Literature summary for 6.3.4.15 extracted from
- Expanding the substrate tolerance of biotin ligase through exploration of enzymes from diverse species (2008), J. Am. Chem. Soc., 130, 1160-1162.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Bacillus subtilis |
| overexpression in Escherichia coli | Homo sapiens |
| overexpression in Escherichia coli | Saccharomyces cerevisiae |
| overexpression in Escherichia coli | Giardia intestinalis |
| overexpression in Escherichia coli | Leuconostoc mesenteroides |
| overexpression in Escherichia coli | Trypanosoma cruzi |
| overexpression in Escherichia coli | Methanocaldococcus jannaschii |
| overexpression in Escherichia coli | Cutibacterium acnes |
| overexpression in Escherichia coli | Pyrococcus horikoshii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bacillus subtilis |  |
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Saccharomyces cerevisiae | |
| Mg2+ | - |
Giardia intestinalis | |
| Mg2+ | - |
Leuconostoc mesenteroides | |
| Mg2+ | - |
Trypanosoma cruzi | |
| Mg2+ | - |
Methanocaldococcus jannaschii | |
| Mg2+ | - |
Cutibacterium acnes | |
| Mg2+ | - |
Pyrococcus horikoshii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Bacillus subtilis | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Escherichia coli | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Homo sapiens | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Saccharomyces cerevisiae | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Giardia intestinalis | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Leuconostoc mesenteroides | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Trypanosoma cruzi | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Methanocaldococcus jannaschii | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Cutibacterium acnes | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Pyrococcus horikoshii | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Cutibacterium acnes | - |
- |
- |
| Escherichia coli | - |
gene birA | - |
| Giardia intestinalis | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Leuconostoc mesenteroides | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
| Pyrococcus horikoshii | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Bacillus subtilis |
| recombinant enzyme from Escherichia coli | Saccharomyces cerevisiae |
| recombinant enzyme from Escherichia coli | Giardia intestinalis |
| recombinant enzyme from Escherichia coli | Leuconostoc mesenteroides |
| recombinant enzyme from Escherichia coli | Trypanosoma cruzi |
| recombinant enzyme from Escherichia coli | Methanocaldococcus jannaschii |
| recombinant enzyme from Escherichia coli | Cutibacterium acnes |
| recombinant enzyme from Escherichia coli | Pyrococcus horikoshii |
| recombinant enzyme from Escherichia coli, the human enzyme degrades significantly during isolation | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Bacillus subtilis | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Escherichia coli | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Homo sapiens | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Saccharomyces cerevisiae | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Giardia intestinalis | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Leuconostoc mesenteroides | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Trypanosoma cruzi | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Methanocaldococcus jannaschii | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Cutibacterium acnes | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Pyrococcus horikoshii | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + biotin + protein p67 | - |
Bacillus subtilis | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Escherichia coli | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Homo sapiens | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Saccharomyces cerevisiae | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Leuconostoc mesenteroides | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Trypanosoma cruzi | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Methanocaldococcus jannaschii | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Cutibacterium acnes | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + biotin + protein p67 | - |
Pyrococcus horikoshii | AMP + diphosphate + biotinyl-protein p67 | - |
? | |
| ATP + cis-propargyl biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Pyrococcus horikoshii | AMP + diphosphate + cis-propargyl bioti-containing [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + cis-propargyl biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Saccharomyces cerevisiae | AMP + diphosphate + cis-propargyl biotin-containing [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| ATP + desthiobiotin azide + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Pyrococcus horikoshii | AMP + diphosphate + desthiobiotin azide-containing [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
| additional information | no activity with protein p67 | Giardia intestinalis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| biotin ligase | - |
Bacillus subtilis |
| biotin ligase | - |
Escherichia coli |
| biotin ligase | - |
Homo sapiens |
| biotin ligase | - |
Saccharomyces cerevisiae |
| biotin ligase | - |
Giardia intestinalis |
| biotin ligase | - |
Leuconostoc mesenteroides |
| biotin ligase | - |
Trypanosoma cruzi |
| biotin ligase | - |
Methanocaldococcus jannaschii |
| biotin ligase | - |
Cutibacterium acnes |
| biotin ligase | - |
Pyrococcus horikoshii |
| BirA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bacillus subtilis |
| 30 | - |
assay at | Escherichia coli |
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 30 | - |
assay at | Giardia intestinalis |
| 30 | - |
assay at | Leuconostoc mesenteroides |
| 30 | - |
assay at | Trypanosoma cruzi |
| 30 | - |
assay at | Methanocaldococcus jannaschii |
| 30 | - |
assay at | Cutibacterium acnes |
| 30 | - |
assay at | Pyrococcus horikoshii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
| ATP | - |
Escherichia coli | |
| ATP | - |
Homo sapiens | |
| ATP | - |
Saccharomyces cerevisiae | |
| ATP | - |
Giardia intestinalis | |
| ATP | - |
Leuconostoc mesenteroides | |
| ATP | - |
Trypanosoma cruzi | |
| ATP | - |
Methanocaldococcus jannaschii | |
| ATP | - |
Cutibacterium acnes | |
| ATP | - |
Pyrococcus horikoshii | |
| biotin | - |
Giardia intestinalis | |
| biotin | specific for | Pyrococcus horikoshii | |
| biotin | highly specific for | Bacillus subtilis | |
| biotin | highly specific for | Escherichia coli | |
| biotin | highly specific for | Homo sapiens | |
| biotin | highly specific for | Saccharomyces cerevisiae | |
| biotin | highly specific for | Leuconostoc mesenteroides | |
| biotin | highly specific for | Trypanosoma cruzi | |
| biotin | highly specific for | Methanocaldococcus jannaschii | |
| biotin | highly specific for | Cutibacterium acnes | |
| cis-propargyl biotin | can substitute for biotin | Saccharomyces cerevisiae | |
| cis-propargyl biotin | can substitute for biotin | Pyrococcus horikoshii | |
| desthiobiotin azide | can substitute for biotin | Pyrococcus horikoshii | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Bacillus subtilis | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Escherichia coli | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Homo sapiens | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Leuconostoc mesenteroides | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Trypanosoma cruzi | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Methanocaldococcus jannaschii | |
| additional information | no activity with biotin analogues desthiobiotin azide, cis-propargyl biotin, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Cutibacterium acnes | |
| additional information | no activity with biotin analogues desthiobiotin azide, trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Saccharomyces cerevisiae | |
| additional information | no activity with biotin analogues trans-propargyl biotin, iminobiotin, diaminobiotin, nitrobenzoxadiazole gamma-butyric acid, iodouracil valeric acid, thiouracil valeric acid | Pyrococcus horikoshii |
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