Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | endproduct inhibition | Escherichia coli | |
MgAMP | endproduct inhibition, mechanism of inhibition, overview | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis | Escherichia coli | |
0.00145 | - |
biotin | pH 8.0, 37°C | Escherichia coli | |
0.25 | - |
ATP | pH 8.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Escherichia coli | biotin protein ligase, BPL required to complete acetyl-CoA carboxylase's capability for fatty acid biosynthesis | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene birA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Escherichia coli | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | biotin protein ligase, BPL required to complete acetyl-CoA carboxylase's capability for fatty acid biosynthesis | Escherichia coli | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
ATP + biotin + fluorescein-labeled peptide 85-11 | peptide 85-11 has the sequence MAGGLNDIFEAQKIEWHE with the lysine residue being targeted for biotinylation, development of a fluorescence polarization technology-based assay method, overview | Escherichia coli | ADP + diphosphate + biotinyl-fluorescein-labeled peptide 85-11 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
biotin protein ligase | - |
Escherichia coli |
BirA | - |
Escherichia coli |
BPL | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
8.24 | - |
pH 8.0, 37°C | Escherichia coli | MgAMP |