Cloned (Comment) | Organism |
---|---|
overexpression of the enzyme comprising residues 1-125 in Escherichia coli strain BL21 | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant native and selenomethionine-labeled enzyme comprising residues 1-125, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
strain OT3 | - |
Pyrococcus horikoshii OT-3 | - |
strain OT3 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme comprising residues 1-125 from Escherichia coli strain BL21 | Pyrococcus horikoshii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine | reaction mechanism involving Lys111, formation of a biotinyl-5'-AMP intermediate, active site structure, ligand binding to the active site loop, overview | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase | Pyrococcus horikoshii | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase | Pyrococcus horikoshii OT-3 | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the functional unit is a dimer, a subunit contains a catalytic N-terminal domain and a C-terminal domain, structure analysis | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
biotin-protein ligase | - |
Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |