Literature summary for 6.3.4.15 extracted from

Bagautdinov, B.; Kuroishi, C.; Sugahara, M.; Kunishima, N.
Purification, crystallization and preliminary crystallographic analysis of the biotin-protein ligase from Pyrococcus horikoshii OT3 (2005), Acta Crystallogr. Sect. F, 61, 193-195.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant native and selenomethionine-labeled enzyme, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution	Pyrococcus horikoshii

Crystallization (Comment)

Organism

purified recombinant native and selenomethionine-labeled enzyme, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution

Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26071	-	2 * 26071 recombinant enzyme, SDS-PAGE and amino acid sequence calculation	Pyrococcus horikoshii
53900	-	recombinant enzyme, light scattering study	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	strain OT3	-
Pyrococcus horikoshii OT-3	-	strain OT3	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21 in a chromatographic procedure comprising several steps	Pyrococcus horikoshii

Subunits

Subunits	Comment	Organism
dimer	2 * 26071 recombinant enzyme, SDS-PAGE and amino acid sequence calculation	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
biotin-protein ligase	-	Pyrococcus horikoshii