Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 17% (w/v) PEG3350 and 0.14 M CaCl2, (in complex with two ADP and two Ca2+ ions), or 0.1 M Bis-tris (pH 6.2), 15% (w/v) PEG3350, 0.1 M NH4Cl, and 5% (w/v) n-octyl-beta-D-glucoside (in complex with two ADP and one Mg2+ ion), or 18% (w/v) PEG3350, 0.17 M CsCl, and 4% (v/v) methanol (R16E mutant in complex with bicarbonate and Mg2+-ADP) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R16E | the mutant has a 2fold loss in catalytic activity compared with the wild type enzyme. The mutation significantly destabilizes the dimer | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Escherichia coli | |
ATP | ATP shows substrate inhibition which is competitive against bicarbonate | Escherichia coli | |
CTP | less than 40% residual activity at 20 mM | Escherichia coli | |
GTP | less than 40% residual activity at 20 mM | Escherichia coli | |
TTP | less than 40% residual activity at 20 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | 2 | ATP | mutant enzyme R16E, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli | |
0.1 | 2 | ATP | wild type enzyme, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli | |
1.9 | - |
HCO3- | wild type enzyme, with 1.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
5 | - |
HCO3- | wild type enzyme, with 7.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
6.5 | - |
HCO3- | wild type enzyme, with 10 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
11.4 | - |
HCO3- | wild type enzyme, with 20 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains Ca2+ | Escherichia coli | |
Mg2+ | dependent on | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | Escherichia coli | - |
ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P24182 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | - |
Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ATP | mutant enzyme R16E, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli | |
0.3 | - |
HCO3- | wild type enzyme, with 20 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
0.31 | - |
HCO3- | wild type enzyme, with 1.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
0.32 | - |
HCO3- | wild type enzyme, with 10 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
0.36 | - |
ATP | wild type enzyme, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli | |
0.36 | - |
HCO3- | wild type enzyme, with 7.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
27 | - |
ATP | wild type enzyme, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the biotin carboxylase subunit of acetyl-CoA carboxylase catalyzes the Mg2+-ATP-dependent carboxylation of biotin | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
HCO3- | wild type enzyme, with 20 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
49 | - |
HCO3- | wild type enzyme, with 10 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
71 | - |
HCO3- | wild type enzyme, with 7.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
162 | - |
HCO3- | wild type enzyme, with 1.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C | Escherichia coli | |
1667 | - |
ATP | mutant enzyme R16E, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli | |
3000 | - |
ATP | wild type enzyme, in 100 mM HEPES (pH 8.0), 5 mM MgCl2, at 30°C | Escherichia coli |