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Literature summary for 6.3.4.14 extracted from

  • Dimroth, P.; Guchhait, R.B.; Stoll, E.; Lane, M.D.
    Enzymatic carboxylation of biotin: molecular and catalytic properties of a component enzyme of acetyl CoA carboxylase (1970), Proc. Natl. Acad. Sci. USA, 67, 1353-1360.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ethanol maximal activation, 11fold at 15% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
ATP
-
Escherichia coli
2.9
-
HCO3-
-
Escherichia coli
170
-
D-(+)-biotin
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
2 * 51000, SDS-PAGE Escherichia coli
95000
-
gel filtration, analytical ultracentrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + HCO3- ATP in form of MgATP2- Escherichia coli ADP + phosphate + carboxybiotin
-
?
ATP + biotin-carboxyl-carrier protein + HCO3-
-
Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
?
ATP + D-(+)-biotin + HCO3-
-
Escherichia coli ADP + phosphate + carboxybiotin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 51000, SDS-PAGE Escherichia coli