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Literature summary for 6.3.4.13 extracted from

  • Shen, Y.; Rudolph, J.M.; Stern, J.R.; Stubbe, J.
    Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization (1990), Biochemistry, 29, 218-227.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
5'-phosphoribosylamine
-
Escherichia coli
0.17
-
ATP
-
Escherichia coli
0.27
-
Gly
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45945
-
x * 45945, calculation from nucleotide sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-phosphoribosylamine + Gly Escherichia coli sequential ordered mechanism of substrate binding and product release in which 5-phosphoribosylamine binds first followed by MgATP2- and then Gly. Phosphate leaves first followed by loss of MgADP- and finally phosphoribosylglycinamide ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-D-ribosylamine + glycine ATP in form of MgATP2- Escherichia coli ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
-
?
ATP + 5-phosphoribosylamine + Gly r Escherichia coli ADP + phosphate + 5-phosphoribosylglycinamide
-
?
ATP + 5-phosphoribosylamine + Gly sequential ordered mechanism of substrate binding and product release in which 5-phosphoribosylamine binds first followed by MgATP2- and then Gly. Phosphate leaves first followed by loss of MgADP- and finally phosphoribosylglycinamide Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 45945, calculation from nucleotide sequence Escherichia coli