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Literature summary for 6.3.4.11 extracted from

  • Höpner, T.; Knappe, J.
    Einbau von Biotin in beta-Methylcrotonyl-CoA-carboxylase durch Holocarboxylase-synthetase (1965), Biochem. Z., 342, 190-206.
    View publication on PubMed
Search for more literature for 6.3.4.11

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Achromobacter sp.

Organism

Organism UniProt Comment Textmining
Achromobacter sp.
-
 IVS-W
-
Achromobacter sp. IVS-W
-
 IVS-W
-
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Achromobacter sp.

Reaction

Reaction Comment Organism Reaction ID
ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] the first reaction step is the reversible formation of biotinyl adenylate, the biotin residue is transferred to the apoenzyme in a second step Achromobacter sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Achromobacter sp.

Storage Stability

Storage Stability Organism
-16°C, stable for several months Achromobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + apo-[3-methylcrotonyl-CoA:carbondioxide ligase (ADP-forming)]
-
 Propionibacterium freudenreichii subsp. shermanii AMP + diphosphate + [3-methylcrotonyl-CoA:carbondioxide ligase (ADP-forming)]
-
 ?
ATP + biotin + apo-[3-methylcrotonyl-CoA:carbondioxide ligase (ADP-forming)] biotin is transferred specifically to the epsilon-amino groups of lysine residues located in apocenters Achromobacter sp. AMP + diphosphate + [3-methylcrotonyl-CoA:carbondioxide ligase (ADP-forming)]
-
 ?
ATP + biotin + apo-[3-methylcrotonyl-CoA:carbondioxide ligase (ADP-forming)] biotin is transferred specifically to the epsilon-amino groups of lysine residues located in apocenters Achromobacter sp. IVS-W AMP + diphosphate + [3-methylcrotonyl-CoA:carbondioxide ligase (ADP-forming)]
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Achromobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8.5 85% of maximal activity at pH 7.4 and 8.5 Achromobacter sp.