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Literature summary for 6.3.4.10 extracted from

  • Rios-Avila, L.; Prince, S.; Wijeratne, S.; Zempleni, J.
    A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity (2011), Clin. Chim. Acta, 412, 735-739.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.10

Application

Application Comment Organism
analysis 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity by biotinylation of the polypeptide p67, which comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669, using IRDye-streptavidin and infrared spectroscopy. The minimal concentration of recombinant HCS that can be detected by this assay is less than 1.08 nmol/l. Jurkat cells contain 0.14 U of HCS activity [in micromol of biotinylated p67 formed/(nmol/l HCS h)] in 400 microg of total protein Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
HCS fused to glutathione S-transferase, S-tag, and both N-terminal and C-terminal 6x His-tags Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1
-
 p67 pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + p67 comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669 Homo sapiens AMP + diphosphate + biotinyl-p67
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