Literature summary for 6.3.3.4 extracted from

Labonte, J.W.; Kudo, F.; Freeman, M.F.; Raber, M.L.; Townsend, C.A.
Engineering the synthetic potential of beta-lactam synthetase and the importance of catalytic loop dynamics (2012), MedChemComm, 3, 960-966.

Search for more literature for 6.3.3.4

Protein Variants

Protein Variants	Comment	Organism
H447A	the mutant shows severely reduced turnover number compared to the wild type enzyme	Streptomyces clavuligerus
V446A	the mutant shows reduced turnover number compared to the wild type enzyme	Streptomyces clavuligerus
V446G	the mutant shows strongly reduced turnover number compared to the wild type enzyme	Streptomyces clavuligerus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.037	-	L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.04	-	(2'R)-methyl-L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.04	-	(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.06	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.06	-	(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.08	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme H447A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.33	-	(2'R)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.36	-	L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.46	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.6	1	L-N2-(2-carboxyethyl)arginine	mutant enzyme V446G, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.9	-	L-N2-(2-carboxyethyl)arginine	mutant enzyme H447A, pH and temperature not specified in the publication	Streptomyces clavuligerus
1.2	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446G, pH and temperature not specified in the publication	Streptomyces clavuligerus
1.9	-	(2'R)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446G, pH and temperature not specified in the publication	Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-N2-(2-carboxyethyl)arginine	Streptomyces clavuligerus	-	AMP + diphosphate + deoxyamidinoproclavaminate	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces clavuligerus	P0DJQ7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (2'R)-methyl-L-N2-(2-carboxyethyl)arginine	-	Streptomyces clavuligerus	?	-	?
ATP + (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine	-	Streptomyces clavuligerus	?	-	?
ATP + (2'S)-methyl-L-N2-(2-carboxyethyl)arginine	-	Streptomyces clavuligerus	?	-	?
ATP + L-N2-(2-carboxyethyl)arginine	-	Streptomyces clavuligerus	AMP + diphosphate + deoxyamidinoproclavaminate	-	?
additional information	no activity with (2'R)-ethyl-L-N2-(2-carboxyethyl)arginine	Streptomyces clavuligerus	?	-	?

Synonyms

Synonyms	Comment	Organism
beta-lactam-synthetase	-	Streptomyces clavuligerus
betaLS	-	Streptomyces clavuligerus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0023	-	(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.004	-	(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.013	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme H447A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.014	-	(2'R)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.016	-	L-N2-(2-carboxyethyl)arginine	mutant enzyme H447A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.023	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.032	-	(2'R)-methyl-L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.07	-	(2'R)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446G, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.13	-	L-N2-(2-carboxyethyl)arginine	mutant enzyme V446G, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.13	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446G, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.28	-	L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.34	-	(2'S)-methyl-L-N2-(2-carboxyethyl)arginine	mutant enzyme V446A, pH and temperature not specified in the publication	Streptomyces clavuligerus
0.87	-	L-N2-(2-carboxyethyl)arginine	wild type enzyme, pH and temperature not specified in the publication	Streptomyces clavuligerus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Streptomyces clavuligerus

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Release 2023.1 (January 2023)