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Literature summary for 6.3.3.4 extracted from

  • Bachmann, B.O.; Townsend, C.A.
    Kinetic mechanism of the beta-Lactam synthetase of Streptomyces clavuligerus (2000), Biochemistry, 39, 11187-11193.
    View publication on PubMed
Search for more literature for 6.3.3.4

Inhibitors

Inhibitors Comment Organism Structure
deoxyamidinoproclavaminate product inhibition Streptomyces clavuligerus
diphosphate product inhibition Streptomyces clavuligerus
N2-(carboxymethyl)-L-arginine competitive to N2-(carboxyethyl)-L-arginine Streptomyces clavuligerus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.153
-
 ATP pH 7.5 Streptomyces clavuligerus
0.22
-
 N2-(carboxyethyl)-L-arginine pH 7.5 Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N2-(carboxyethyl)-L-arginine + ATP Streptomyces clavuligerus early step in clavulanic acid biosynthesis AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus P0DJQ7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein expressed in E. coli Streptomyces clavuligerus

Reaction

Reaction Comment Organism Reaction ID
ATP + L-N2-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate it has been proposed that L-N2-(2-carboxyethyl)arginine is first converted into an acyl-AMP by reaction with ATP and loss of diphosphate, and that the beta-lactam ring is then formed by the intramolecular attack of the beta-nitrogen on the activated carboxy group, in a sequential ordered bi-ter kinetic mechanism Streptomyces clavuligerus
a

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, 10% glycerol, 2 mM dithiothreitol, 0.01 mM EDTA, pH 7.5, stable Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N2-(carboxyethyl)-L-arginine + ATP mechanism Streptomyces clavuligerus AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?
N2-(carboxyethyl)-L-arginine + ATP early step in clavulanic acid biosynthesis Streptomyces clavuligerus AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?