Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, yield of purified recombinant enzyme depends on the growth conditions, M9 media is referred, overview | Mus musculus |
General Stability | Organism |
---|---|
the enzyme is stabilized by Tween 20 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
(6S)-5-formyltetrahydrofolate | pH 6.0, 28°C, recombinant enzyme | Mus musculus | |
0.769 | - |
ATP | recombinant enzyme | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | as MgATP2- | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23200 | - |
x * 23200, recombinant enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | Mus musculus | the enzyme affects intracellular folate concentrations by accelerating folate degradation | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
ATP + 5-formyltetrahydrofolate | Mus musculus 129/Sv | the enzyme affects intracellular folate concentrations by accelerating folate degradation | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
additional information | Mus musculus | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | ? | - |
? | |
additional information | Mus musculus 129/Sv | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9D110 | - |
- |
Mus musculus 129/Sv | Q9D110 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 171fold to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His-tag is removed by thrombin cleavage and dialysis | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.22 | - |
purified recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6S)-5-formyltetrahydrofolate | - |
Mus musculus | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
ATP + (6S)-5-formyltetrahydrofolate | - |
Mus musculus 129/Sv | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
ATP + 5-formyltetrahydrofolate | ATP in form of MgATP2- | Mus musculus | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydrofolate | ATP in form of MgATP2- | Mus musculus 129/Sv | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydrofolate | the enzyme affects intracellular folate concentrations by accelerating folate degradation | Mus musculus | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
ATP + 5-formyltetrahydrofolate | the enzyme affects intracellular folate concentrations by accelerating folate degradation | Mus musculus 129/Sv | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
additional information | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | Mus musculus | ? | - |
? | |
additional information | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | Mus musculus 129/Sv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23200, recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
5,10-Methenyltetrahydrofolate synthetase | - |
Mus musculus |
MTHFS | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 28 | assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.3 | - |
(6S)-5-formyltetrahydrofolate | pH 6.0, 28°C, recombinant enzyme | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on, as MgATP2-, binding site motifs | Mus musculus |