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Literature summary for 6.3.3.2 extracted from

  • Anguera, M.C.; Liu, X.; Stover, P.J.
    Cloning, expression, and purification of 5,10-methenyltetrahydrofolate synthetase from Mus musculus (2004), Protein Expr. Purif., 35, 276-283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, yield of purified recombinant enzyme depends on the growth conditions, M9 media is referred, overview Mus musculus

General Stability

General Stability Organism
the enzyme is stabilized by Tween 20 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
(6S)-5-formyltetrahydrofolate pH 6.0, 28°C, recombinant enzyme Mus musculus
0.769
-
ATP recombinant enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ as MgATP2- Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23200
-
x * 23200, recombinant enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-formyltetrahydrofolate Mus musculus the enzyme affects intracellular folate concentrations by accelerating folate degradation ADP + 5,10-methenyltetrahydrofolate + phosphate
-
?
ATP + 5-formyltetrahydrofolate Mus musculus 129/Sv the enzyme affects intracellular folate concentrations by accelerating folate degradation ADP + 5,10-methenyltetrahydrofolate + phosphate
-
?
additional information Mus musculus disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects ?
-
?
additional information Mus musculus 129/Sv disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9D110
-
-
Mus musculus 129/Sv Q9D110
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 171fold to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His-tag is removed by thrombin cleavage and dialysis Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.22
-
purified recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6S)-5-formyltetrahydrofolate
-
Mus musculus ADP + 5,10-methenyltetrahydrofolate + phosphate
-
?
ATP + (6S)-5-formyltetrahydrofolate
-
Mus musculus 129/Sv ADP + 5,10-methenyltetrahydrofolate + phosphate
-
?
ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Mus musculus ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?
ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Mus musculus 129/Sv ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?
ATP + 5-formyltetrahydrofolate the enzyme affects intracellular folate concentrations by accelerating folate degradation Mus musculus ADP + 5,10-methenyltetrahydrofolate + phosphate
-
?
ATP + 5-formyltetrahydrofolate the enzyme affects intracellular folate concentrations by accelerating folate degradation Mus musculus 129/Sv ADP + 5,10-methenyltetrahydrofolate + phosphate
-
?
additional information disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects Mus musculus ?
-
?
additional information disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects Mus musculus 129/Sv ?
-
?

Subunits

Subunits Comment Organism
? x * 23200, recombinant enzyme, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
5,10-Methenyltetrahydrofolate synthetase
-
Mus musculus
MTHFS
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24 28 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.3
-
(6S)-5-formyltetrahydrofolate pH 6.0, 28°C, recombinant enzyme Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on, as MgATP2-, binding site motifs Mus musculus