Cloned (Comment) | Organism |
---|---|
gene FAU1, orf YER183c, functional expression as His-tagged enzyme in Escherichia coliBL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption mutants do not evoke an altered phenotype | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP- | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
5-formyltetrahydrofolate | recombinant enzyme, pH 7.0, 30°C | Saccharomyces cerevisiae | |
0.043 | - |
ATP | recombinant enzyme, pH 7.0, 30°C | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | Saccharomyces cerevisiae | - |
ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in disruption mutant strains | Saccharomyces cerevisiae |
7 | - |
purified recombinant enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | - |
Saccharomyces cerevisiae | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydrofolate | - |
Saccharomyces cerevisiae | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
Methenyltetrahydrofolate synthetase | - |
Saccharomyces cerevisiae |
MTHFS | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
MgATP2- | enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP- | Saccharomyces cerevisiae |