Literature summary for 6.3.3.2 extracted from

Huennekens, F.M.; Henderson, G.B.; Vitols, K.S.; Grimshaw, C.E.
Enzymatic activation of 5-formyltetrahydrofolate via conversion to 5,10-methenyltetrahydrofolate (1984), Adv. Enzyme Regul., 22, 3-13.

Search for more literature for 6.3.3.2

General Stability

General Stability	Organism
enzyme loses activity when subjected to dialysis or concentration by ultrafiltration	Lacticaseibacillus casei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	5-formyltetrahydrofolate	-	Lacticaseibacillus casei
0.001	-	ATP	-	Lacticaseibacillus casei

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Oryctolagus cuniculus
Mg2+	required	Ovis aries
Mg2+	required	Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	gel filtration	Lacticaseibacillus casei
25000	-	gel filtration	Oryctolagus cuniculus
26500	29000	gel filtration, HPLC gel filtration, equilibrium ultracentrifugation	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-
Lacticaseibacillus casei	-	-	-
Mus musculus	-	-	-
Oryctolagus cuniculus	-	-	-
Ovis aries	-	-	-

Oxidation Stability

Oxidation Stability	Organism
rapid loss of activity unless kept in the presence of reducing agents, e.g. DTT	Lacticaseibacillus casei

Purification (Commentary)

Purification (Comment)	Organism
-	Lacticaseibacillus casei

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate	mechanism is proposed to involve phosphorylation of the formyl group to create an enol phosphate	Lacticaseibacillus casei	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
colonic cancer cell line	-	Mus musculus	-
L-1210 cell	L210 mouse leukemia cells	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.8	-	-	Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5-formyltetrahydrofolate	-	Gallus gallus	ADP + phosphate + 5,10-methylenetetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	-	Mus musculus	ADP + phosphate + 5,10-methylenetetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	-	Oryctolagus cuniculus	ADP + phosphate + 5,10-methylenetetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	-	Ovis aries	ADP + phosphate + 5,10-methylenetetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	-	Lacticaseibacillus casei	ADP + phosphate + 5,10-methylenetetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	ATP in form of MgATP2-	Lacticaseibacillus casei	ADP + phosphate + 5,10-methenyltetrahydrofolate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 25000, SDS-PAGE	Oryctolagus cuniculus
monomer	1 * 23000, SDS-PAGE	Lacticaseibacillus casei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Oryctolagus cuniculus
additional information	-	additional information	-	Lacticaseibacillus casei

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Release 2023.1 (January 2023)