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Literature summary for 6.3.2.B4 extracted from
- TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1 (2008), FEBS Lett., 582, 1129-1134.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged TTLL10 in Spodoptera frugiperda Sf9 cells, co-expression of EGFP-tagged TTLL10 with FLAG-tagged NAP1 in HEK-293 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E499V | site-directed mutagenesis, inactive active site mutant | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-tubulin + glycine | Mus musculus | - |
glycyl-alpha-tubulin + ADP + phosphate | - |
? | |
| ATP + nucleosome assembly protein 1 + glycine | Mus musculus | i.e. NAP1, glycylation at Glu residues at 359 and 360 | glycylated nucleosome assembly protein 1 + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged TTLL10 from Spodoptera frugiperda Sf9 cells, co-expression of EGFP-tagged TTLL10 with FLAG-tagged NAP1 in HEK-293 cells | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | tissue distribution study, overview | Mus musculus | - |
| spermatid | immunohistochemic enzyme determination | Mus musculus | - |
| testis | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-tubulin + glycine | - |
Mus musculus | glycyl-alpha-tubulin + ADP + phosphate | - |
? | |
| ATP + nucleosome assembly protein 1 + glycine | i.e. NAP1, glycylation at Glu residues at 359 and 360 | Mus musculus | glycylated nucleosome assembly protein 1 + ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycine ligase | - |
Mus musculus |
| More | polyglycylase belongs to family of tubulin tyrosine ligase-like, TTLL, proteins | Mus musculus |
| polyglycylase | - |
Mus musculus |
| protein polyglycylase | - |
Mus musculus |
| TTLL10 | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | absolutely dependent on | Mus musculus | |
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Release 2023.1 (January 2023)
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