Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D317A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
D35A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
E157A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
E157K | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
H183A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
H301A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
K115A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
K198A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
K198F | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
N268A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
N271A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
R302A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
R425A | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
Y194F | reduced enzymatic activity with respect to the wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, N268A mutant protein | Escherichia coli | |
0.016 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, N271A mutant protein | Escherichia coli | |
0.03 | - |
ATP | pH 9.4, 37°C, E157A mutant protein | Escherichia coli | |
0.04 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, H301A mutant protein | Escherichia coli | |
0.044 | - |
D-glutamate | pH 9.4, 37°C, D317A mutant protein | Escherichia coli | |
0.046 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, K115A mutant protein | Escherichia coli | |
0.053 | - |
D-glutamate | pH 9.4, 37°C, N271A mutant protein | Escherichia coli | |
0.053 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, R425A mutant protein | Escherichia coli | |
0.053 | - |
D-glutamate | pH 9.4, 37°C, Y194F mutant protein | Escherichia coli | |
0.056 | - |
D-glutamate | pH 9.4, 37°C, E157K mutant protein | Escherichia coli | |
0.057 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, D317A mutant protein | Escherichia coli | |
0.059 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, H183A mutant protein | Escherichia coli | |
0.065 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, R302A mutant protein | Escherichia coli | |
0.074 | - |
D-glutamate | pH 9.4, 37°C, R302A mutant protein | Escherichia coli | |
0.084 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, D35A mutant protein | Escherichia coli | |
0.085 | - |
ATP | pH 9.4, 37°C, E157K mutant protein | Escherichia coli | |
0.095 | - |
D-glutamate | pH 9.4, 37°C, D35A mutant protein | Escherichia coli | |
0.095 | - |
D-glutamate | pH 9.4, 37°C, H301A mutant protein | Escherichia coli | |
0.111 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, Y194F mutant protein | Escherichia coli | |
0.114 | - |
ATP | pH 9.4, 37°C, D35A mutant protein | Escherichia coli | |
0.12 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, K198A mutant protein | Escherichia coli | |
0.128 | - |
D-glutamate | pH 9.4, 37°C, K115A mutant protein | Escherichia coli | |
0.129 | - |
ATP | pH 9.4, 37°C, K198F mutant protein | Escherichia coli | |
0.13 | - |
ATP | pH 9.4, 37°C, H183A mutant protein | Escherichia coli | |
0.135 | - |
ATP | pH 9.4, 37°C, K198A mutant protein | Escherichia coli | |
0.14 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, K198F mutant protein | Escherichia coli | |
0.14 | - |
ATP | pH 9.4, 37°C, N268A mutant protein | Escherichia coli | |
0.144 | - |
ATP | pH 9.4, 37°C, Y194F mutant protein | Escherichia coli | |
0.153 | - |
ATP | pH 9.4, 37°C, H301A mutant protein | Escherichia coli | |
0.183 | - |
ATP | pH 9.4, 37°C, R425A mutant protein | Escherichia coli | |
0.185 | - |
D-glutamate | pH 9.4, 37°C, K198A mutant protein | Escherichia coli | |
0.216 | - |
D-glutamate | pH 9.4, 37°C, K198F mutant protein | Escherichia coli | |
0.218 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, E157K mutant protein | Escherichia coli | |
0.264 | - |
D-glutamate | pH 9.4, 37°C, N268A mutant protein | Escherichia coli | |
0.304 | - |
UDP-N-acetylmuramoyl L-alanine | pH 9.4, 37°C, E157A mutant protein | Escherichia coli | |
0.328 | - |
ATP | pH 9.4, 37°C, K115A mutant protein | Escherichia coli | |
0.806 | - |
D-glutamate | pH 9.4, 37°C, H183A mutant protein | Escherichia coli | |
1.58 | - |
ATP | pH 9.4, 37°C, N271A mutant protein | Escherichia coli | |
2 | - |
ATP | pH 9.4, 37°C, R302A mutant protein | Escherichia coli | |
2.18 | - |
ATP | pH 9.4, 37°C, D317A mutant protein | Escherichia coli | |
5.16 | - |
D-glutamate | pH 9.4, 37°C, R425A mutant protein | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain DH5alpha | - |
Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
r | |
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate | - |
Escherichia coli DH5-alpha | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
D-glutamate | pH 9.4, 37°C, E157K mutant protein | Escherichia coli | |
0.0004 | - |
D-glutamate | pH 9.4, 37°C, H183A mutant protein | Escherichia coli | |
0.004 | - |
D-glutamate | pH 9.4, 37°C, K115A mutant protein | Escherichia coli | |
0.012 | - |
D-glutamate | pH 9.4, 37°C, K198F mutant protein | Escherichia coli | |
0.016 | - |
D-glutamate | pH 9.4, 37°C, K198A mutant protein | Escherichia coli | |
0.022 | - |
D-glutamate | pH 9.4, 37°C, D317A mutant protein | Escherichia coli | |
0.093 | - |
D-glutamate | pH 9.4, 37°C, Y194F mutant protein | Escherichia coli | |
0.3 | - |
D-glutamate | pH 9.4, 37°C, N271A mutant protein | Escherichia coli | |
0.53 | - |
D-glutamate | pH 9.4, 37°C, R425A mutant protein | Escherichia coli | |
0.77 | - |
D-glutamate | pH 9.4, 37°C, H301A mutant protein | Escherichia coli | |
3 | 6 | D-glutamate | pH 9.4, 37°C, R302A mutant protein | Escherichia coli | |
3.13 | - |
D-glutamate | pH 9.4, 37°C, D35A mutant protein | Escherichia coli | |
3.13 | - |
D-glutamate | pH 9.4, 37°C, R302A mutant protein | Escherichia coli | |
3.3 | - |
D-glutamate | pH 9.4, 37°C, N268A mutant protein | Escherichia coli | |
4.27 | - |
D-glutamate | pH 9.4, 37°C, D35A mutant protein | Escherichia coli | |
5.12 | - |
D-glutamate | pH 9.4, 37°C, N268A mutant protein | Escherichia coli | |
6.08 | - |
D-glutamate | pH 9.4, 37°C, H301A mutant protein | Escherichia coli | |
6.08 | - |
D-glutamate | pH 9.4, 37°C, R425A mutant protein | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
- |
Escherichia coli |