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Literature summary for 6.3.2.4 extracted from

  • Wu, D.; Kong, Y.; Han, C.; Chen, J.; Hu, L.; Jiang, H.; Shen, X.
    D-Alanine:D-alanine ligase as a new target for the flavonoids quercetin and apigenin (2008), Int. J. Antimicrob. Agents, 32, 421-426.
    View publication on PubMed
Search for more literature for 6.3.2.4

Inhibitors

Inhibitors Comment Organism Structure
apigenin 4',5,7-trihydroxyflavone, inhibition mechanism, competitive with respect to the substrate ATP and non-competitive to the substrate D-Ala Escherichia coli
apigenin 4',5,7-trihydroxyflavone, inhibition mechanism, competitive with respect to the substrate ATP and non-competitive to the substrate D-Ala Helicobacter pylori
quercetin i.e. 3,3',4',5,7-pentahydroxyflavone, inhibition mechanism, competitive with respect to the substrate ATP and non-competitive to the substrate D-Ala Escherichia coli
quercetin i.e. 3,3',4',5,7-pentahydroxyflavone, inhibition mechanism, competitive with respect to the substrate ATP and non-competitive to the substrate D-Ala Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis Escherichia coli
additional information
-
 additional information kinetic analysis Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
Mg2+
-
 Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-alanine Escherichia coli
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?
ATP + D-alanine Helicobacter pylori
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strains BL21(DE3) and JM109
-
Helicobacter pylori
-
 strains SS1 and ATCC 43504, ddl gene
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-alanine
-
 Escherichia coli ADP + phosphate + D-alanyl-D-alanine
-
 ?
ATP + D-alanine
-
 Helicobacter pylori ADP + phosphate + D-alanyl-D-alanine
-
 ?

Synonyms

Synonyms Comment Organism
D-Alanine:D-alanine ligase
-
 Escherichia coli
D-Alanine:D-alanine ligase
-
 Helicobacter pylori
Ddl
-
 Helicobacter pylori
DdlB
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli
25
-
 assay at Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Escherichia coli
7.4
-
 assay at Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Helicobacter pylori

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Helicobacter pylori
additional information
-
 additional information inhibition kinetics at pH 7.4 and 25°C Escherichia coli
0.0043
-
 quercetin pH 7.4, 25°C, versus ATP Helicobacter pylori
0.0097
-
 quercetin pH 7.4, 25°C, versus ATP Escherichia coli
0.0101
-
 quercetin pH 7.4, 25°C, versus D-Ala Escherichia coli
0.028
-
 quercetin pH 7.4, 25°C, versus D-Ala Helicobacter pylori
0.031
-
 apigenin pH 7.4, 25°C, versus ATP Helicobacter pylori
0.0807
-
 apigenin pH 7.4, 25°C, versus ATP Escherichia coli
0.085
-
 apigenin pH 7.4, 25°C, versus D-Ala Helicobacter pylori
0.1195
-
 apigenin pH 7.4, 25°C, versus D-Ala Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0199
-
 pH 7.4, 25°C Escherichia coli quercetin
0.0485
-
 pH 7.4, 25°C Helicobacter pylori quercetin
0.1327
-
 pH 7.4, 25°C Helicobacter pylori apigenin
0.163
-
 pH 7.4, 25°C Escherichia coli apigenin