Cloned (Comment) | Organism |
---|---|
expressed as C-terminal His6-tagged enzyme in Escherichia coli DH5alpha cells | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP-N-acetylmuramoyl-L-Ala-D-Glu | strong inhibition by excess UDP-N-acetylmuramoyl-L-Ala-D-Glu is observed | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the optimum Mg2+ concentration for L-lysine adding activity is 15 mM | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP | D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine | Thermotoga maritima | UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate | - |
? | |
UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP | D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate | - |
? | |
UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys + ATP | - |
Thermotoga maritima | UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + ADP + phosphate | - |
? | |
UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid + ATP | meso-diaminopimelic acid is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 10% efficiency relative to L-lysine | Thermotoga maritima | UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurE | - |
Thermotoga maritima |
MurE synthetase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
temperature optimum for L-lysine adding activity. At 37°C, the activity is about 25% of the optimal value | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
for L-lysine adding activity | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermotoga maritima |