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Literature summary for 6.3.2.35 extracted from
- Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase (1997), Proc. Natl. Acad. Sci. USA, 94, 10040-10044.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Enterococcus casseliflavus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
D-serine | pH 7.5 | Enterococcus casseliflavus |  |
| 1.9 | - |
ATP | pH 7.5 | Enterococcus casseliflavus | |
| 7.2 | - |
D-asparagine | pH 7.5 | Enterococcus casseliflavus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus casseliflavus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Enterococcus casseliflavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + D-2-aminobutanoate + ATP | - |
Enterococcus casseliflavus | D-alanyl-D-2-aminobutanoate + ADP + phosphate | - |
? | |
| D-alanine + D-alanine + ATP | formation of D-alanyl-D-serine is 400fold prefered over formation of D-alanyl-D-alanine | Enterococcus casseliflavus | D-alanyl-D-alanine + ADP + phosphate | - |
? | |
| D-alanine + D-allo-threonine + ATP | - |
Enterococcus casseliflavus | D-alanyl-D-allo-threonine + ADP + phosphate | - |
? | |
| D-alanine + D-asparagine + ATP | - |
Enterococcus casseliflavus | D-alanyl-D-asparagine + ADP + phosphate | - |
? | |
| D-alanine + D-glutamine + ATP | - |
Enterococcus casseliflavus | D-alanyl-D-glutamine + ADP + phosphate | - |
? | |
| D-alanine + D-homoserine + ATP | - |
Enterococcus casseliflavus | D-alanyl-D-homoserine + ADP + phosphate | - |
? | |
| D-alanine + D-serine + ATP | formation of D-alanyl-D-serine is 400fold prefered over formation of D-alanyl-D-alanine | Enterococcus casseliflavus | D-alanyl-D-serine + ADP + phosphate | - |
? | |
| D-alanine + D-threonine + ATP | - |
Enterococcus casseliflavus | D-alanyl-D-threonine + ADP + phosphate | - |
? | |
| additional information | amino acid preference in decreasing order: D-Ser, D-Asn, D-Thr, D-Gln, D-homoserine, D-aminobutanoate, D-allo-Thr | Enterococcus casseliflavus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VanC2 | - |
Enterococcus casseliflavus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.17 | - |
D-serine | pH 7.5 | Enterococcus casseliflavus | |
| 8.87 | - |
D-asparagine | pH 7.5 | Enterococcus casseliflavus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | broad | Enterococcus casseliflavus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.23 | - |
D-asparagine | pH 7.5 | Enterococcus casseliflavus | |
| 4.5 | - |
D-serine | pH 7.5 | Enterococcus casseliflavus | |
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