Cloned (Comment) | Organism |
---|---|
overexpression in an Escherichia coli expression system | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | product inhibition | Arabidopsis thaliana | |
glutathione | product inhibition | Arabidopsis thaliana | |
phosphate | product inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
gamma-Glu-L-Cys | 25°C, pH 7.5 | Arabidopsis thaliana | |
0.057 | - |
ATP | 25°C, pH 7.5 | Arabidopsis thaliana | |
1.51 | - |
Gly | 25°C, pH 7.5 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P46416 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-Glu-L-Cys + Gly | random ter-reactant mechanism, ADP is the last product released | Arabidopsis thaliana | ADP + phosphate + glutathione | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.1 | - |
ATP | 25°C, pH 7.5 | Arabidopsis thaliana | |
12.2 | - |
gamma-Glu-L-Cys | 25°C, pH 7.5 | Arabidopsis thaliana | |
12.6 | - |
Gly | 25°C, pH 7.5 | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
ADP | 25°C, pH 7.5, with glycine as varied substrate | Arabidopsis thaliana | |
1.89 | - |
ADP | 25°C, pH 7.5, with ATP as varied substrate | Arabidopsis thaliana | |
2.67 | - |
ADP | 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate | Arabidopsis thaliana | |
16.6 | - |
glutathione | 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate | Arabidopsis thaliana | |
22.7 | - |
glutathione | 25°C, pH 7.5, with glycine as varied substrate | Arabidopsis thaliana | |
25.1 | - |
phosphate | 25°C, pH 7.5, with ATP as varied substrate | Arabidopsis thaliana | |
35.4 | - |
glutathione | 25°C, pH 7.5, with ATP as varied substrate | Arabidopsis thaliana | |
47.3 | - |
phosphate | 25°C, pH 7.5, with glycine as varied substrate | Arabidopsis thaliana | |
69.1 | - |
phosphate | 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate | Arabidopsis thaliana |