Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.3 extracted from

  • Jez, J.M.; Cahoon, R.E.
    Kinetic mechanism of glutathione synthetase from Arabidopsis thaliana (2004), J. Biol. Chem., 279, 42726-42731.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in an Escherichia coli expression system Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
ADP product inhibition Arabidopsis thaliana
glutathione product inhibition Arabidopsis thaliana
phosphate product inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
gamma-Glu-L-Cys 25°C, pH 7.5 Arabidopsis thaliana
0.057
-
ATP 25°C, pH 7.5 Arabidopsis thaliana
1.51
-
Gly 25°C, pH 7.5 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P46416
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly random ter-reactant mechanism, ADP is the last product released Arabidopsis thaliana ADP + phosphate + glutathione
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.1
-
ATP 25°C, pH 7.5 Arabidopsis thaliana
12.2
-
gamma-Glu-L-Cys 25°C, pH 7.5 Arabidopsis thaliana
12.6
-
Gly 25°C, pH 7.5 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.77
-
ADP 25°C, pH 7.5, with glycine as varied substrate Arabidopsis thaliana
1.89
-
ADP 25°C, pH 7.5, with ATP as varied substrate Arabidopsis thaliana
2.67
-
ADP 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate Arabidopsis thaliana
16.6
-
glutathione 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate Arabidopsis thaliana
22.7
-
glutathione 25°C, pH 7.5, with glycine as varied substrate Arabidopsis thaliana
25.1
-
phosphate 25°C, pH 7.5, with ATP as varied substrate Arabidopsis thaliana
35.4
-
glutathione 25°C, pH 7.5, with ATP as varied substrate Arabidopsis thaliana
47.3
-
phosphate 25°C, pH 7.5, with glycine as varied substrate Arabidopsis thaliana
69.1
-
phosphate 25°C, pH 7.5, with gamma-Glu-L-Cys as varied substrate Arabidopsis thaliana