Activating Compound | Comment | Organism | Structure |
---|---|---|---|
activator protein 1 | i.e. AP-1, mediates the induction of enzyme expression by tert-butylhydroquinone, binds to the enzyme promotor, antagonisted by c-jun, a dominant negative which is also produced in enhanced levels after treatment with tert-butylhydroquinone | Rattus norvegicus | |
tert-butylhydroquinone | regulatory role, activator protein 1 AP-1-mediated induction of enzyme expression, 35fold increase in activity in recombinant H4IIE cells, induction of c-jun formation, which is a negative regulatory protein blocking the binding of activatot protein 1 to the promotor | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
cloning of the 2.2 kb 5'-flanking region, DNA sequence determination and analysis, promotor determination, determination of positive and negative regulation regions, e.g. NF1 repressor for induction of enzyme expression by tert-butylhydroquinone, expression in H4IIE cells, ATCC CRL-1548, via transformation by recombinant adenovirus vector | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
c-jun | negative regulation of enzyme expression by blocking the binding of AP-1 to the promotor | Rattus norvegicus | |
tert-butylhydroquinone | regulatory role, activator protein 1 AP-1-mediated induction of enzyme expression, 35fold increase in activity in recombinant H4IIE cells, induction of c-jun formation, which is a negative regulatory protein blocking the binding of activator protein 1 to the promotor | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-L-Cys + Gly | Rattus norvegicus | - |
ADP + phosphate + glutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | AF333982 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-L-Cys + Gly | - |
Rattus norvegicus | ADP + phosphate + glutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glutathione synthetase | - |
Rattus norvegicus |
GS | - |
Rattus norvegicus |