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Literature summary for 6.3.2.3 extracted from

  • Moran, J.F.; Iturbe-Ormaetxe, I.; Matamoros, M.A.; Rubio, M.C.; Clemente, M.R.; Brewin, N.J.; Becana, M.
    Glutathione and homoglutathione synthetases of legume nodules. Cloning, expression, and subcellular localization (2000), Plant Physiol., 124, 1381-1392.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.3

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination Pisum sativum
DNA and amino acid sequence determination Phaseolus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme GSHS2 Pisum sativum 5829
-
cytosol isozyme GSHS2 Phaseolus vulgaris 5829
-
cytosol of nodule bacteroid and leaf Vigna unguiculata 5829
-
mitochondrion isozyme GSHS1 Pisum sativum 5739
-
mitochondrion of nodule Vigna unguiculata 5739
-
plastid isozyme GSHS2 Phaseolus vulgaris 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly Vigna unguiculata
-
 ADP + phosphate + gamma-glutathione
-
 ir
ATP + gamma-Glu-L-Cys + Gly Pisum sativum
-
 ADP + phosphate + gamma-glutathione
-
 ir
ATP + gamma-Glu-L-Cys + Gly Phaseolus vulgaris
-
 ADP + phosphate + gamma-glutathione
-
 ir

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris Q9FV26 isozyme GSHS2, strain 3622
-
Pisum sativum Q9FV27 isozyme GSHS2
-
Pisum sativum Q9FVA2 isozyme GSHS1
-
Vigna unguiculata
-
 cowpea, strain 32H1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information isozyme GSHS2 contains no signal peptide Pisum sativum
proteolytic modification isozyme GSHS1 precursor contains a signal peptide of about 20 amino acid length, cleavage site is FFSKH-/-IPST Pisum sativum
proteolytic modification isozyme GSHS2 precursor contains a signal peptide of about 59 amino acid length, cleavage site is NSAPL-/-AEPD Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Vigna unguiculata
-
leaf
-
 Pisum sativum
-
additional information isozyme GSHS2 is not expressed in leaves Pisum sativum
-
additional information isozyme GSHS2 is not expressed in leaves Phaseolus vulgaris
-
nodule
-
 Pisum sativum
-
nodule bacteroid Vigna unguiculata
-
nodule bacteroid Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Vigna unguiculata
additional information
-
-
 Pisum sativum
additional information
-
-
 Phaseolus vulgaris
0.0006
-
 crude extract and cytosolic fraction of leaves Vigna unguiculata
0.0008
-
 mitochondrial fraction of leaves Vigna unguiculata
0.0094
-
 bacteroid isolate Phaseolus vulgaris
0.0127
-
 bacteroid isolate Vigna unguiculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly
-
 Vigna unguiculata ADP + phosphate + gamma-glutathione
-
 ir
ATP + gamma-Glu-L-Cys + Gly
-
 Pisum sativum ADP + phosphate + gamma-glutathione
-
 ir
ATP + gamma-Glu-L-Cys + Gly
-
 Phaseolus vulgaris ADP + phosphate + gamma-glutathione
-
 ir

Synonyms

Synonyms Comment Organism
Glutathione synthetase
-
 Vigna unguiculata
Glutathione synthetase
-
 Phaseolus vulgaris
Glutathione synthetase
-
 Pisum sativum
GSHS
-
 Vigna unguiculata
GSHS
-
 Phaseolus vulgaris
GSHS
-
 Pisum sativum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Vigna unguiculata
ATP
-
 Pisum sativum
ATP
-
 Phaseolus vulgaris

pI Value

Organism Comment pI Value Maximum pI Value
Pisum sativum isozyme GSHS2, isoelectric focusing
-
 5.5
Phaseolus vulgaris isozyme GSHS2 precursor, isoelectric focusing 5.9 5.6
Pisum sativum isozyme GSHS1 precursor, isoelectric focusing
-
 6.5