Literature summary for 6.3.2.3 extracted from

Frendo, P.; Jimenez, M.J.; Mathieu, C.; Duret, L.; Gallesi, D.; Van de Sype, G.; Herouart, D.; Puppo, A.
A Medicago truncatula homoglutathione synthetase is derived from glutathione synthetase by gene duplication (2001), Plant Physiol., 126, 1706-1715.

Search for more literature for 6.3.2.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene gshs1, construction of cDNA library, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain 830, phylogenetic analysis reveals that gshs1 and gshs2, encoding homoglutathione synthetase, are a result of gene duplication, genomic organization	Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + gamma-L-Glu-L-Cys + Gly	Medicago truncatula	-	ADP + phosphate + glutathione	-	?

Organism

Organism	UniProt	Comment	Textmining
Medicago truncatula	Q94GA0	gene gshs1, cv. Jemalong genotype J5	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione	residues A534 and A535 are involved in substrate binding and determination of substrate specificity	Medicago truncatula	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Medicago truncatula

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + gamma-L-Glu-L-Cys + Gly	-	Medicago truncatula	ADP + phosphate + glutathione	-	?

Synonyms

Synonyms	Comment	Organism
Glutathione synthetase	-	Medicago truncatula
GS	-	Medicago truncatula
GSHS1	-	Medicago truncatula

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Medicago truncatula

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Release 2023.1 (January 2023)