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Literature summary for 6.3.2.3 extracted from

  • Tanaka, T.; Yamaguchi, H.; Kato, H.; Nishioka, T.; Katsube, Y.; Oda, J.
    Flexibility impaired by mutations related the multifunctional roles of the loop in glutathione synthetase (1993), Biochemistry, 32, 12398-12404.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region Escherichia coli

Protein Variants

Protein Variants Comment Organism
G240V mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region Escherichia coli
P227V mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region Escherichia coli
P227V/G240V mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region Escherichia coli

General Stability

General Stability Organism
the remaining activity after arginyl-endopeptidase treatment is higher in the presence of ATP and/or gamma-Glu-Cys than in their absence Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values for Gly and gamma-Glu-Cys are sensitive to the mutation and drastically increased Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 wild-type and mutant enzymes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli ADP + phosphate + glutathione
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover number is fatally reduced in the P227V/G240V mutant Escherichia coli