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Literature summary for 6.3.2.3 extracted from

  • Oppenheimer, L.; Wellner, V.P.; Griffith, O.W.; Meister.A.
    Glutathione synthetase. Purification from rat kidney and mapping of the substrate binding sites (1979), J. Biol. Chem., 254, 5184-5190.
    View publication on PubMed
Search for more literature for 6.3.2.3

General Stability

General Stability Organism
loss of activity by exposure to Mn2+ for long periods Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) inhibition of ADP-ATP exchange reaction, no effect on tripeptide synthesis Rattus norvegicus
NEM inhibition of ADP-ATP exchange reaction, no effect on tripeptide synthesis Rattus norvegicus
PCMB
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 ATP
-
 Rattus norvegicus
0.76
-
 Gly
-
 Rattus norvegicus
25
-
 N-acetyl-L-Cys
-
 Rattus norvegicus
60
-
 N-acetyl-L-2-aminobutyrate
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 77% of the efficiency relative to Mg2+ Rattus norvegicus
K+ no requirement for exchange reaction Rattus norvegicus
K+ strict requirement for monovalent cation, maximal activity with K+ Rattus norvegicus
Li+ can replace K+ Rattus norvegicus
Mg2+ divalent metal ion required, Mg2+ is most effective Rattus norvegicus
Mn2+ 73% of the efficiency relative to Mg2+ Rattus norvegicus
NH4+ can replace K+ Rattus norvegicus
Rb+ can replace K+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 2 * 59000, SDS-PAGE Rattus norvegicus
118000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains about 2% carbohydrate Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
stable for more than 2 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-Cys + Gly
-
 Rattus norvegicus ADP + phosphate + acetyl-Cys-Gly
-
 ?
ATP + beta-aminoglutaryl-L-2-aminobutyrate + Gly 15% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + beta-aminoglutaryl-L-2-aminobutyryl-Gly
-
 ?
ATP + DL-gamma-(beta-methyl)Glu-L-2-aminobutyrate + Gly 44% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + DL-gamma-(beta-methyl)Glu-L-2-aminobutyryl-Gly
-
 ?
ATP + DL-gamma-(gamma-methyl)Glu-L-2-aminobutyrate + Gly 16% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + DL-gamma-(gamma-methyl)Glu-L-2-aminobutyryl-Gly
-
 ?
ATP + gamma-Glu-aminobutyrate + Gly L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + ophthalmic acid
-
 ?
ATP + gamma-Glu-aminobutyrate + Gly D-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + ophthalmic acid
-
 ?
ATP + gamma-Glu-L-Cys + aminooxyacetate 53% of the activity relative to Gly Rattus norvegicus ADP + phosphate + gamma-Glu-Cys-aminoxyacetate
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Rattus norvegicus ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + N-hydroxyglycine 33% of the activity relative to Gly Rattus norvegicus ADP + phosphate + gamma-Glu-Cys-N-hydroxyglycine
-
 ?
ATP + gamma-Glu-S-methylcysteine + Gly 24% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + gamma-Glu-S-methylcysteinyl-Gly
-
 ?
ATP + Glu-L-2-aminobutyrate + Gly 16% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + gamma-Glu-L-Cys-Gly
-
 ?
ATP + L-gamma-(alpha-methyl)Glu-L-2-aminobutyrate + Gly
-
 Rattus norvegicus ADP + phosphate + L-gamma-(alpha-methyl)Glu-L-2-aminobutyryl-Gly
-
 ?
ATP + L-gamma-(N-methyl)Glu-L-2-aminobutyrate + Gly 52% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + L-gamma-(N-methyl)Glu-L-2-aminobutyryl-Gly
-
 ?
ATP + L-gamma-Glu-Gly + Gly 8% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + L-gamma-Glu-Gly-Gly
-
 ?
ATP + L-gamma-Glu-L-2-aminopentanoic acid + Gly 9% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + gamma-Glu-S-methylcysteinyl-Gly
-
 ?
ATP + L-gamma-Glu-L-Ala + Gly 55% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + L-gamma-Glu-L-Ala-Gly
-
 ?
ATP + L-gamma-Glu-L-Cys + Gly 102% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + L-gamma-Glu-L-Cys-Gly
-
 ?
ATP + L-gamma-Glu-L-Ser + Gly 77% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + L-gamma-Glu-L-Ser-Gly
-
 ?
ATP + N-acetyl-L-2-aminobutyrate + Gly 8% of the activity relative to L-gamma-glutamyl-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + N-acetyl-L-2-aminobutyrate-Gly
-
 ?
ATP + N-acetyl-L-Cys + Gly 20% of the activity relative to L-gamma-Glu-L-2-aminobutyrate Rattus norvegicus ADP + phosphate + N-acetyl-L-Cys-Gly
-
 ?
CTP + gamma-Glu-L-Cys + Gly 4% of the activity relative to ATP Rattus norvegicus CDP + phosphate + glutathione
-
 ?
dATP + gamma-Glu-L-Cys + Gly 75% of the activity relative to ATP Rattus norvegicus dADP + phosphate + glutathione
-
 ?
GTP + gamma-Glu-L-Cys + Gly 3% of the activity relative to ATP Rattus norvegicus GDP + phosphate + glutathione
-
 ?
ITP + gamma-Glu-L-Cys + Gly 6% of the activity relative to ATP Rattus norvegicus IDP + phosphate + glutathione
-
 ?
additional information the enzyme catalyzes ADP-ATP exchange at 0.62% of the rate of tripeptide synthesis Rattus norvegicus ?
-
 ?
UTP + gamma-Glu-L-Cys + Gly 24% of the activity relative to ATP Rattus norvegicus UDP + phosphate + glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 59000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
 5 min, stable in absence of glutathione or other thiols Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9.7 about 50% of maximal activity at pH 7.0 and 9.7 Rattus norvegicus