Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TOP10 cells | Geminocystis herdmanii PCC 6308 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Synechocystis sp. PCC 6804 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from Synechocystis and from recombinant Escherichia coli cells | Geminocystis herdmanii PCC 6308 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre | Geminocystis herdmanii PCC 6308 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0091 | - |
enzyme isolated from Synechocystis cells, 28°C | Geminocystis herdmanii PCC 6308 |
0.027 | - |
recombinant enzyme isolated from Escherichia coli | Geminocystis herdmanii PCC 6308 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n + L-Asp | [L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase | Geminocystis herdmanii PCC 6308 | ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | no formation of AMP | ? |
Synonyms | Comment | Organism |
---|---|---|
CphA | - |
Geminocystis herdmanii PCC 6308 |
cyanophycin synthetase | - |
Geminocystis herdmanii PCC 6308 |