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Literature summary for 6.3.2.26 extracted from

  • Ishibashi, T.; Tomita, H.; Yokooji, Y.; Morikita, T.; Watanabe, B.; Hiratake, J.; Kishimoto, A.; Kita, A.; Miki, K.; Imanaka, T.; Atomi, H.
    A detailed biochemical characterization of phosphopantothenate synthetase, a novel enzyme involved in coenzyme A biosynthesis in the Archaea (2012), Extremophiles, 16, 819-828.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichoa coli Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
4-phosphopantoate substrate inhibition Thermococcus kodakarensis
ATP substrate inhibition Thermococcus kodakarensis
additional information feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
beta-Alanine pH 6.5, 85°C Thermococcus kodakarensis
1.16
-
(R)-4-phosphopantoate pH 6.5, 85°C Thermococcus kodakarensis
2.44
-
ATP pH 6.5, 85°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-4-phosphopantoate + beta-alanine Thermococcus kodakarensis the enzyme is involved in coenzyme A biosynthesis in the archaea AMP + diphosphate + (R)-4'-phosphopantothenate
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JIZ8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.38
-
beta-alanine, pH 6.5, 85°C Thermococcus kodakarensis
14.7
-
ATP, pH 6.5, 85°C Thermococcus kodakarensis
15.6
-
(R)-4-phosphopantoate, pH 6.5, 85°C Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-4-phosphopantoate + beta-alanine the enzyme is involved in coenzyme A biosynthesis in the archaea Thermococcus kodakarensis AMP + diphosphate + (R)-4'-phosphopantothenate
-
?
ATP + (R)-4-phosphopantoate + beta-alanine strict preference for ATP. Among several amine substrates, activity is detected with beta-alanine, but not with gamma-aminobutyrate, glycine nor aspartate Thermococcus kodakarensis AMP + diphosphate + (R)-4'-phosphopantothenate
-
?

Synonyms

Synonyms Comment Organism
phosphopantothenate synthetase
-
Thermococcus kodakarensis
Pps
-
Thermococcus kodakarensis
TK1686
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
assay at Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 95 activity continues to increase with temperature elevation from 65°C up to 95°C Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
6 h, no decrease in activity Thermococcus kodakarensis
80
-
half-life: 13.6 h Thermococcus kodakarensis
90
-
half-life: 8.2 h Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Thermococcus kodakarensis