Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Streptomyces clavuligerus |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | stability of crude enzyme is increased by dithiothreitol | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminoadipate + L-cysteine + L-valine + ATP | - |
Streptomyces clavuligerus | N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + AMP + diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Streptomyces clavuligerus |
25 | - |
half of activity after 8 h | Streptomyces clavuligerus |
32 | - |
half of activity after 10 min | Streptomyces clavuligerus |
34 | - |
the mixture of ATP 5 mM, Mg2+ 5 mM, valine 5 mM prevents the thermal inactivation, 92% activity after 10 min | Streptomyces clavuligerus |
34 | - |
the mixture of ATP 5 mM, DDT 3 mM, Mg2+ 5 mM, aminoadipate 5 mM, cysteine 1 mM and valine 5 mM prevents the thermal inactivation, 91% activity after 10 min | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptomyces clavuligerus |