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Literature summary for 6.3.2.26 extracted from

  • Zhang, J.; Demain, A.L.
    In vitro stabilization of ACV synthetase activity from Streptomyces clavuligerus (1992), Appl. Biochem. Biotechnol., 37, 97-110.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Streptomyces clavuligerus

Organic Solvent Stability

Organic Solvent Comment Organism
dithiothreitol stability of crude enzyme is increased by dithiothreitol Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminoadipate + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + AMP + diphosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
-
Streptomyces clavuligerus
25
-
half of activity after 8 h Streptomyces clavuligerus
32
-
half of activity after 10 min Streptomyces clavuligerus
34
-
the mixture of ATP 5 mM, Mg2+ 5 mM, valine 5 mM prevents the thermal inactivation, 92% activity after 10 min Streptomyces clavuligerus
34
-
the mixture of ATP 5 mM, DDT 3 mM, Mg2+ 5 mM, aminoadipate 5 mM, cysteine 1 mM and valine 5 mM prevents the thermal inactivation, 91% activity after 10 min Streptomyces clavuligerus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Streptomyces clavuligerus