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Literature summary for 6.3.2.26 extracted from

  • Zhang, J.; Wolfe, S.; Demain, A.L.
    Biochemical studies on the activity of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus (1992), Biochem. J., 283, 691-698.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoate 1 mM Streptomyces clavuligerus
AMP 5 mM Streptomyces clavuligerus
ATP at concentrations about 5 mM Streptomyces clavuligerus
diphosphate 5 mM Streptomyces clavuligerus
EDTA 10 mM Streptomyces clavuligerus
iodoacetamide 1 mM Streptomyces clavuligerus
N-ethylmaleimide 1 mM Streptomyces clavuligerus
pyridoxal 5'-phosphate 2 mM Streptomyces clavuligerus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-cysteine
-
Streptomyces clavuligerus
0.3
-
L-valine
-
Streptomyces clavuligerus
0.63
-
L-aminoadipate
-
Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 10 mM Streptomyces clavuligerus

Organic Solvent Stability

Organic Solvent Comment Organism
dithiothreitol 8 mM leads to a 10% higher enzyme activity than at 3 mM Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0113
-
-
Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminoadipate + L-cysteine + L-allo-isoleucine + ATP
-
Streptomyces clavuligerus L-delta-(aminoadipyl)-L-cysteinyl-D-allo-isoleucine + AMP + diphosphate
-
?
L-2-aminoadipate + L-cysteine + L-isoleucine + ATP
-
Streptomyces clavuligerus L-delta-(aminoadipyl)-L-cysteinyl-D-isoleucine + AMP + diphosphate
-
?
L-2-aminoadipate + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + AMP + diphosphate
-
ir
L-2-aminoadipate + L-homocysteine + L-valine + ATP
-
Streptomyces clavuligerus N-(5-amino-5-carboxypentanoyl)-L-homocysteinyl-D-valine + AMP + diphosphate
-
?
L-glutamate + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus L-glutamyl-L-cysteinyl-D-valine + AMP + diphosphate
-
?
S-carboxymethylcysteine + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus L-S-carboxymethylcysteinyl-L-cysteinyl-D-valine + AMP + diphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
29.5
-
-
Streptomyces clavuligerus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 32
-
Streptomyces clavuligerus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half of activity after 70 h Streptomyces clavuligerus
25
-
half of activity after 10 h Streptomyces clavuligerus
32
-
half of activity after 10 min Streptomyces clavuligerus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Streptomyces clavuligerus

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Streptomyces clavuligerus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
very unstable below pH 7 Streptomyces clavuligerus
8.5
-
very unstable above pH 8.5 Streptomyces clavuligerus

Cofactor

Cofactor Comment Organism Structure
ATP optimal concentration: 1.5-5 mM Streptomyces clavuligerus