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Literature summary for 6.3.2.25 extracted from

  • Wehland, J.; Schröder, H.C.; Weber, K.
    Isolation and purification of tubulin tyrosine ligase (1986), Methods Enzymol., 134, 170-179.
    View publication on PubMed
Search for more literature for 6.3.2.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0254
-
 L-Tyr
-
 Sus scrofa
0.432
-
 L-Phe
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, SDS-PAGE Sus scrofa
43000
-
 glycerol gradient centrifugation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-70°C, protein concentration 0.02 mg/ml, 20-40% glycerol, stable for at least 1 year Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + detyrosinated alpha-tubulin + L-DOPA
-
 Sus scrofa ?
-
 ?
ATP + detyrosinated alpha-tubulin + L-Phe
-
 Sus scrofa ?
-
 ?
ATP + detyrosinated alpha-tubulin + L-Tyr
-
 Sus scrofa alpha-tubulin + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Sus scrofa