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Literature summary for 6.3.2.23 extracted from

  • Macnicol, P.K.
    Homoglutathione and glutathione synthetases of legume seedlings: partial purification and substrate specificity (1987), Plant Sci., 53, 229-235.
No PubMed abstract available
Search for more literature for 6.3.2.23

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate 5 mM: 10-30% inhibition Vigna radiata var. radiata
ADP 5 mM: 70% inhibition Vigna radiata var. radiata
AMP 5 mM: 10-30% inhibition Vigna radiata var. radiata
cysteine 5 mM: 15% inhibition Vigna radiata var. radiata
glutathione 5 mM: 15% inhibition Vigna radiata var. radiata
additional information methionine, homocysteine, S-adenosyl-methionine, serine, all 5 mM, are no inhibitors Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 beta-Alanine
-
 Vigna radiata var. radiata
0.45
-
 ATP
-
 Vigna radiata var. radiata
0.75
-
 gamma-glutamylcysteine
-
 Vigna radiata var. radiata
7.5
-
 glycine
-
 Vigna radiata var. radiata
8.3
-
 gamma-aminobutyrate
-
 Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Vigna radiata var. radiata
Mg2+ 2-3 mM optimal Vigna radiata var. radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 gel filtration Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine Vigna radiata var. radiata
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homoglutathione ?

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Vigna radiata var. radiata

Source Tissue

Source Tissue Comment Organism Textmining
aerial part
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.042
-
 gamma-glutamylcysteine + beta-alanine Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
-20°C, more than 1 mg enzyme/ml in potassium phosphate buffer, 30-50% glycerol, 1 mM dithiothreitol, stable for at least 4 months Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-glutamylcysteine + gamma-aminobutyrate
-
 Vigna radiata var. radiata ADP + phosphate + gamma-glutamylcysteinyl-4-aminobutyrate
-
 ?
ATP + gamma-glutamylcysteine + glycine
-
 Vigna radiata var. radiata ADP + phosphate + gamma-glutamylcysteinylglycine
-
 ?
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Vigna radiata var. radiata ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homoglutathione ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Vigna radiata var. radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 20 h, 50 mM potassium phosphate buffer, pH 7.5: 15% remaining activity. 20 h, buffer plus 10% (v/v) glycerol: 30% remaining activity. 20 h, buffer with glycerol plus 1 mM dithiothreitol: 50% remaining activity. 20 h, buffer with glycerol and dithiothreitol plus 10 mg/ml bovine serum albumin: 85% remaining activity Vigna radiata var. radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
7 10 50% of maximal activity at pH 7 and pH 10 Vigna radiata var. radiata