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Literature summary for 6.3.2.2 extracted from

  • Mukherjee, A.; Roy, G.; Guimond, C.; Ouellette, M.
    The gamma-glutamylcysteine synthetase gene of Leishmania is essential and involved in response to oxidants (2009), Mol. Microbiol., 74, 914-927.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
antimony heterozygous mutants with one allele inactivated show a significant decreased survival in the presence of antimony Leishmania infantum
oxidative stress heterozygous mutants with one allele inactivated are more susceptible to oxidative stresses in vitro as promastigotes and show decreased survival inside activated macrophages producing reactive oxygen or nitrogen species Leishmania infantum

Organism

Organism UniProt Comment Textmining
Leishmania infantum
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General Information

General Information Comment Organism
physiological function generation of GSH1 null mutants in Leishmania infantum. Removal of even a single wild-type allelic copy of GSH1 invariably leads to the generation of an extra copy of GSH1, maintaining two intact wild-type alleles. By first supplementing the parasites with a rescue plasmid, both a single and null chromosomal GSH1 mutant can be obtained. Parasites with one intact GSH1 chromosomal allele lose the rescuing plasmid but not the double knockout, when grown in the absence of antibiotic, indicating the essentiality of the GSH1 gene. Heterozygous mutants with one allele inactivated transcribe less GSH1 mRNA and synthesize less glutathione and trypanothione. These mutants are more susceptible to oxidative stresses in vitro as promastigotes and show decreased survival inside activated macrophages producing reactive oxygen or nitrogen species. These mutants show a significant decreased survival in the presence of antimony Leishmania infantum