Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Yang, Y.; Chen, Y.; Johansson, E.; Schneider, S.N.; Shertzer, H.G.; Nebert, D.W.; Dalton, T.P.
    Interaction between the catalytic and modifier subunits of glutamate-cysteine ligase (2007), Biochem. Pharmacol., 74, 372-381.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C248A/C249A mutant enzyme shows the same strength of binding to regulatory subunit (GCLM) as does wild-type GCLC, yet the catalytic activity is dramatically decreased Mus musculus
additional information deletion analysis indicats that most regions, except a portion of the C-terminal region of catalytic subunit (GCLC) and a portion of the N-terminal region of regulatory subunit (GCLM), are required for the interaction to occur Mus musculus
P158L mutant enzyme shows the same strength of binding to regulatory subunit (GCLM) as does wild-type GCLC, yet the catalytic activity is dramatically decreased Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
GSH
-
Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Mus musculus rate-limiting enzyme in the glutathione biosynthesis pathway ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine rate-limiting enzyme in the glutathione biosynthesis pathway Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Subunits

Subunits Comment Organism
dimer heterodimer, comprising a catalytic subunit (GCLC) and a regulatory subunit (GCLM). GCLC alone can catalyze the formation of L-gamma-glutamyl-L-cysteine, its binding with GCLM enhances the enzyme activity by lowering the Km for glutamate and ATP, and increasing the Ki for GSH inhibition Mus musculus