KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
cysteine | enzyme from embryo homogenate and from visceral yolk sac homogenate | Rattus norvegicus | |
0.75 | - |
glutamate | enzyme from embryo homogenate | Rattus norvegicus | |
1.38 | - |
glutamate | enzyme from visceral yolk sac homogenate | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | Rattus norvegicus | rate-limiting enzyme in GSH synthesis | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | basal expression of glutamate-cysteine ligase catalytic subunit and regulatory subunit is 59fold and 25fold higher in visceral yolk sac, respectively, compared to the embryo | Rattus norvegicus | - |
yolk sac | visceral | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | - |
Rattus norvegicus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | rate-limiting enzyme in GSH synthesis | Rattus norvegicus | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |