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Literature summary for 6.3.2.2 extracted from

  • Ashida, H.; Sawa, Y.; Shibata, H.
    Cloning, biochemical and phylogenetic characterizations of gamma-glutamylcysteine synthetase from Anabaena sp. PCC 7120 (2005), Plant Cell Physiol., 46, 557-562.
    View publication on PubMed
Search for more literature for 6.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nostoc sp.

Inhibitors

Inhibitors Comment Organism Structure
glutathione
-
 Nostoc sp.
L-buthionine sulfoximine
-
 Nostoc sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-cysteine pH 8.4, 25°C Nostoc sp.
0.23
-
 ATP pH 8.4, 25°C Nostoc sp.
0.82
-
 L-glutamate pH 8.4, 25°C Nostoc sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Nostoc sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Nostoc sp. first step of glutathione synthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
 PCC 7120
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
 Nostoc sp. ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine first step of glutathione synthesis Nostoc sp. ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Nostoc sp.

Synonyms

Synonyms Comment Organism
gamma-GCS
-
 Nostoc sp.
gamma-Glutamylcysteine synthetase
-
 Nostoc sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.5
-
 glutathione
-
 Nostoc sp.
29.3
-
 L-buthionine sulfoximine
-
 Nostoc sp.