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Literature summary for 6.3.2.2 extracted from

  • Vergauwen, B.; De Vos, D.; Van Beeumen, J.J.
    Characterization of the bifunctional gamma -glutamate-cysteine ligase-glutathione synthetase (GshF) of Pasteurella multocida (2005), J. Biol. Chem., 281, 4380-4394.
    View publication on PubMed
Search for more literature for 6.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pasteurella multocida

Inhibitors

Inhibitors Comment Organism Structure
glutathione feedback inhibition Pasteurella multocida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 L-cysteine pH 8.2, 25°C Pasteurella multocida
0.22
-
 L-Cys 25°C, pH 8.2 Pasteurella multocida
0.25
-
 ATP pH 8.2, 25°C Pasteurella multocida
0.25
-
 ATP 25°C, pH 8.2 Pasteurella multocida
5.3
-
 L-glutamate pH 8.2, 25°C Pasteurella multocida
5.3
-
 L-Glu 25°C, pH 8.2 Pasteurella multocida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 2 * 85000, SDS-PAGE Pasteurella multocida
191000
-
 bifunctional enzyme gamma-glutamate-cysteine ligase-glutathione synthetase, gel filtration Pasteurella multocida
220000
-
 gel filtration Pasteurella multocida

Organism

Organism UniProt Comment Textmining
Pasteurella multocida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pasteurella multocida

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, can be stored for up to 6 months without significant loss of activity Pasteurella multocida
-80°C, purified recombinant GshF in 50 mM Tris-HCl, pH 7.4, containing about 100 mM NaCl could be stored for up to 6 months without significant loss of activity Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
 Pasteurella multocida ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 85000, SDS-PAGE Pasteurella multocida
dimer 2 *85000, SDS-PAGE Pasteurella multocida

Synonyms

Synonyms Comment Organism
gamma-ECL
-
 Pasteurella multocida
gamma-glutamate-cysteine ligase-glutathione synthetase bifunctional enzyme Pasteurella multocida
gamma-glutamate-cysteine ligase/glutathione synthetase bifunctional enzyme Pasteurella multocida
GshF bifunctional enzyme gamma-glutamate-cysteine ligase-glutathione synthetase Pasteurella multocida
GshF bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase Pasteurella multocida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.4
-
 ATP pH 8.2, 25°C Pasteurella multocida
22.4
-
 ATP 25°C, pH 8.2 Pasteurella multocida
24.3
-
 L-glutamate pH 8.2, 25°C Pasteurella multocida
24.3
-
 L-Glu 25°C, pH 8.2 Pasteurella multocida
25.3
-
 L-cysteine pH 8.2, 25°C Pasteurella multocida
25.3
-
 L-Cys 25°C, pH 8.2 Pasteurella multocida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
13.6
-
 glutathione
-
 Pasteurella multocida
13.6
-
 glutathione 25°C, pH 8.2 Pasteurella multocida