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Literature summary for 6.3.2.2 extracted from

  • Gegg, M.E.; Clark, J.B.; Heales, S.J.R.
    Determination of glutamate-cysteine ligase (gamma-glutamylcysteine synthetase) activity by high-performance liquid chromatography and electrochemical detection (2002), Anal. Biochem., 304, 26-32.
    View publication on PubMed
Search for more literature for 6.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
GSH
-
 Rattus norvegicus
L-buthionine-S-sulfoximine specific inhibitor Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte primary cell culture Rattus norvegicus
-
cell culture astrocyte, primary Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0097
-
 partially purified enzyme from astrocytes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Synonyms

Synonyms Comment Organism
gamma-GCS
-
 Rattus norvegicus
gamma-Glutamylcysteine synthetase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 L-buthionine-S-sulfoximine about, pH 8.2, 37°C Rattus norvegicus
8.2
-
 GSH pH 8.2, 37°C Rattus norvegicus