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Literature summary for 6.3.2.2 extracted from
- Escherichia coli B gamma-glutamylcysteine synthetase: modification, purification, crystallization and preliminary crystallographic analysis (2002), Acta Crystallogr. Sect. D, 58, 316-318.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme is a target for development of potential therapeutic agents | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli JM109 | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 22.6 mg/ml purified enzyme, wild-type or mutant, hanging drop vapour diffusion method, equal volume of protein and reservoir solution, reservoir solution: 3.9 M sodium formate, pH 7.4, equilibration with reservoir solution at 293K, X-ray diffraction structure determination and analysis at 2.8 A resolution | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C106S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
| C164S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
| C205S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
| C223S | site-directed mutagenesis, exchange of surface exposed cysteine residue for improved crystallization | Escherichia coli |
| additional information | construction of a quadruple mutant of the enzyme termed gamma-GCS4CS | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | Escherichia coli | catalyzes the biosynthesis of the GSH precursor | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |  |
| ATP + L-Glu + L-Cys | Escherichia coli B / ATCC 11303 | catalyzes the biosynthesis of the GSH precursor | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B | - |
| Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | - |
Escherichia coli | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-Cys | catalyzes the biosynthesis of the GSH precursor | Escherichia coli | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-Cys | - |
Escherichia coli B / ATCC 11303 | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-Cys | catalyzes the biosynthesis of the GSH precursor | Escherichia coli B / ATCC 11303 | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-GCS | - |
Escherichia coli |
| gamma-Glutamylcysteine synthetase | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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