Cloned (Comment) | Organism |
---|---|
cDNA for heavy subunit expressed in Escherichia coli | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gamma-Glu-2-aminobutanoyl-Gly | i.e. ophthalmic acid, inhibits only slightly, but inhibits much more after treatment of the holoenzyme with DTT, the recombinant and isolated heavy subunit enzyme is substantially inhibited without DTT | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-Cys | L-Cys | Rattus norvegicus | |
0.8 | - |
L-2-aminobutanoate | recombinant heavy subunit | Rattus norvegicus | |
1.2 | - |
L-Glu | L-2-aminobutanoate | Rattus norvegicus | |
1.4 | - |
L-2-aminobutanoate | L-Glu, holoenzyme | Rattus norvegicus | |
18.2 | - |
L-Glu | recombinant heavy subunit | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27700 | - |
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
73000 | - |
1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT | Rattus norvegicus |
More | the light subunit has a regulatory function affecting the affinity for Glu and GSH | Rattus norvegicus |