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Literature summary for 6.3.2.2 extracted from
- gamma-Glutamylcysteine synthetase (rat kidney) (1971), Methods Enzymol., 17B, 495-500.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Rattus norvegicus |  |
| NEM | - |
Rattus norvegicus | |
| PCMB | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
L-Cys | ATP, holoenzyme and recombinant heavy subunit | Rattus norvegicus | |
| 1.3 | - |
L-2-aminobutanoate | - |
Rattus norvegicus | |
| 1.6 | - |
L-Glu | - |
Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 50% loss of activity after 6-7 days, only 5% of activity remains after 1 month, substrates protect, almost complete reactivation by addition of 2-5 mM | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + DL-allylglycine | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-DL-allylglycine | - |
? | |
| ATP + L-Glu + L-2-aminobutanoate | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-2-aminobutanoate | - |
? | |
| ATP + L-Glu + L-Ala | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Ala | - |
? | |
| ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-homocysteine | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-homocysteine | - |
? | |
| ATP + L-Glu + L-Thr | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Thr | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.4 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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