Cloned (Comment) | Organism |
---|---|
the folC gene from Escherichia coli is cloned into plasmid pET29. The resulting plasmid, pVRC1432, is transferred into the Escherichia coli BL21 strain | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop method in 1.2-1.7 M ammonium sulfate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in Escherichia coli, the addition of L-glutamate to dihydropteroate (dihydrofolate synthetase activity) and the subsequent additions of L-glutamate to tetrahydrofolate (folylpolyglutamate synthetase (FPGS) activity) are catalyzed by the same enzyme, FolC. The presence of a folate binding site in Escherichia coli FolC, which is different from the one seen in folylpolyglutamate synthetases, provides avenues for the design of specific inhibitors of this enzyme in antimicrobial therapy | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
FolC | - |
Escherichia coli |