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Literature summary for 6.3.2.17 extracted from

  • Turner, F.B.; Andreassi 2nd, J.L.; Ferguson, J.; Titus, S.; Tse, A.; Taylor, S.M.; Moran, R.G.
    Tissue-specific expression of functional isoforms of mouse folypoly-gamma-glutamate synthetase: a basis for targeting folate antimetabolites (1999), Cancer Res., 59, 6074-6079.
    View publication on PubMed
Search for more literature for 6.3.2.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
mitochondrion
-
 Mus musculus 5739
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the enzyme catalyzes the formation of polyglutamates from folates and folate antimetabolites in mammalian cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
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 mouse
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Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephacryl S-100/HR and DEAE-Sephacel chromatography, recombinant enzymes Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
L-1210 cell tumor, leukemic cells Mus musculus
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liver there is a difference in the primary sequence of this isoform with respect to the leukemic cells isoform Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-R 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate at similar rate as that seen with aminopterin as substrate, endogenous enzyme isolated from liver. More efficient than aminopterin, recombinant liver enzyme Mus musculus ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
 ?
ATP + 6-S 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate more efficient than aminopterin, recombinant liver enzyme Mus musculus ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
 ?
ATP + aminopterin + L-glutamate more efficient than 6-S 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 and 6-R 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2, recombinant enzyme from leukemic cells Mus musculus ?
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 ?
additional information the enzyme catalyzes the formation of polyglutamates from folates and folate antimetabolites in mammalian cells Mus musculus ?
-
 ?