KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
5,6,7,8-tetrahydropteroyl-Glu | - |
Datura inoxia | |
35.7 | - |
ATP | - |
Datura inoxia | |
150 | - |
L-Glu | - |
Datura inoxia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Datura inoxia | |
Mg2+ | maximal activity at 20 mM | Datura inoxia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Datura inoxia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Datura inoxia | enzyme of folate biosynthetic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura inoxia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Datura inoxia |
Storage Stability | Organism |
---|---|
stable at 0-4°C or at -20°C | Datura inoxia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate | - |
Datura inoxia | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | n: 1 | Datura inoxia | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu | - |
Datura inoxia | ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2 | - |
? | |
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu | n: 1 | Datura inoxia | ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
ATP + 7,8-dihydropteroyl-Glun + Glu | n: 1 | Datura inoxia | ADP + phosphate + 7,8-dihydropteroyl-Glun+1 | - |
? | |
ATP + methotrexate + Glu | - |
Datura inoxia | ADP + phosphate + methotrexyl-Glu | - |
? | |
additional information | enzyme of folate biosynthetic pathway | Datura inoxia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, SDS-PAGE | Datura inoxia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Datura inoxia |