Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Reducing agents | absolutely dependent on | Homo sapiens | |
Reducing agents | beta-mercaptoethanol or DTT | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
7,8-dihydropteroyl-Glu | - |
Homo sapiens | |
0.002 | - |
5,6,7,8-tetrahydropteroyl-Glu4 | 5,6,7,8-tetrahydropteroyl-Glu | Homo sapiens | |
0.0037 | - |
10-formyl-5,6,7,8-tetrahydropteroyl-Glu | - |
Homo sapiens | |
0.0044 | - |
5,6,7,8-tetrahydropteroyl-Glu | aminopterin | Homo sapiens | |
0.048 | - |
7,8-dihydropteroyl-Glu2 | - |
Homo sapiens | |
0.06 | - |
pteroyl-Glu | - |
Homo sapiens | |
0.081 | - |
methotrexate | - |
Homo sapiens | |
0.105 | - |
5-formyl-5,6,7,8-tetrahydropteroyl-Glu | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | absolutely dependent on monovalent cation, K+ is most effective | Homo sapiens | |
Mn2+ | stimulates | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate | - |
Homo sapiens | ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2 | - |
? | |
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | n: 1 | Homo sapiens | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu | - |
Homo sapiens | ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2 | - |
? | |
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate | - |
Homo sapiens | ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2 | - |
? | |
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu | n: 1 | Homo sapiens | ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
ATP + 7,8-dihydropteroyl-Glu + L-glutamate | - |
Homo sapiens | ADP + phosphate + 7,8-dihydropteroyl-gamma-Glu2 | - |
? | |
ATP + 7,8-dihydropteroyl-Glun + Glu | n: 1 | Homo sapiens | ADP + phosphate + 7,8-dihydropteroyl-Glun+1 | - |
? | |
ATP + aminopterin + Glu | - |
Homo sapiens | ADP + phosphate + aminopteryl-Glu | - |
? | |
ATP + methotrexate + Glu | - |
Homo sapiens | ADP + phosphate + methotrexyl-Glu | - |
? | |
ATP + pteroyl-Glu + Glu | n: 1, i.e. pteroylmonoglutamate, folic acid | Homo sapiens | ADP + phosphate + pteroylmonoglutamyl-Glu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Homo sapiens |