Any feedback?
Literature summary for 6.3.2.17 extracted from
- Folylpolyglutamate synthase from higher plants (1997), Methods Enzymol., 281, 141-145.
General Stability
| General Stability | Organism |
|---|---|
| 50% glycerol stabilizes for several days at 4°C | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
5,6,7,8-tetrahydropteroyl-Glu | - |
Pisum sativum |  |
| 0.61 | - |
L-Glu | - |
Pisum sativum | |
| 2.3 | - |
ATP | - |
Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | cotyledon | Pisum sativum | 5829 | - |
| mitochondrion | leaf | Pisum sativum | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaHCO3 | optimal activation at 10 mM | Homo sapiens | |
| NaHCO3 | optimal activation at 10 mM | Pisum sativum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Pisum sativum | - |
| leaf | - |
Pisum sativum | - |
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme loses activity within 1-2 days at 4, without stabilizer | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu | n:1 | Pisum sativum | ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
| ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate | - |
Pisum sativum | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2 | - |
? | |
| ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | - |
Homo sapiens | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
| ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | - |
Pisum sativum | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | 7.4: about 50% of maximal activity | Pisum sativum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
