Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D392A | kcat lower than wild-type, Km (meso-2,6-diaminoheptanedioate) and Km (ATP) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by at least partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100% | Mycobacterium tuberculosis |
DELTA1-24 | N-terminal deletion mutant, kcat comparable to wild-type, Km values comparable to wild-type, mutant shows similar results to the wild-type with all the nucleotides, UDP-MurNAc peptides and amino acids | Mycobacterium tuberculosis |
E220A | kcat lower than wild-type, Km (ATP) and Km (UDP-MurNAc-L-Ala-D-Glu), Km (meso-2,6-diaminoheptanedioate) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by atleast partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100% | Mycobacterium tuberculosis |
K157A | kcat lower than wild-type, Km (meso-2,6-diaminoheptanedioate), Km (UDP-MurNAc-L-Ala-D-Glu) and Km (ATP) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by at least partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100% | Mycobacterium tuberculosis |
N449D | kcat lower than wild-type, Km (ATP) and Km (meso-2,6-diaminoheptanedioate) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, mutant shows similar results to the wild-type with all the nucleotides, UDP-MurNAc peptides and amino acids, except D-Lys, which shows a slightly higher activity for mutant N449D | Mycobacterium tuberculosis |
R451A | kcat lower than wild-type, Km (ATP) comparable to wild-type, Km (meso-2,6-diaminoheptanedioate) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, significant activity in the presence of L-Lys, D-Lys, DL-ornithine and D-Glu for mutant R451A with similar activities as wild type for all the nucleotides and UDP-MurNAc peptides tested | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
UDP-MurNAc-L-Ala-D-Glu | mutant K157A, pH 8.5, 37°C, value below | Mycobacterium tuberculosis | |
0.005 | - |
UDP-MurNAc-L-Ala-D-Glu | mutant R451A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.007 | - |
UDP-MurNAc-L-Ala-D-Glu | mutant N449D, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.008 | - |
ATP | mutant K157A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.012 | - |
meso-2,6-diaminoheptanedioate | mutant K157A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.015 | - |
ATP | mutant R451A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.016 | - |
UDP-MurNAc-L-Ala-D-Glu | mutant D392A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.017 | - |
ATP | wild-type, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.019 | - |
UDP-MurNAc-L-Ala-D-Glu | DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.021 | - |
ATP | DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.024 | - |
UDP-MurNAc-L-Ala-D-Glu | wild-type, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.052 | - |
meso-2,6-diaminoheptanedioate | mutant E220A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.06 | - |
ATP | mutant N449D, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.065 | - |
meso-2,6-diaminoheptanedioate | DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.078 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.1 | - |
ATP | mutant D392A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.113 | - |
meso-2,6-diaminoheptanedioate | mutant D392A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.123 | - |
ATP | mutant E220A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.148 | - |
UDP-MurNAc-L-Ala-D-Glu | mutant E220A, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.324 | - |
meso-2,6-diaminoheptanedioate | mutant N449D, pH 8.5, 37°C | Mycobacterium tuberculosis | |
0.473 | - |
meso-2,6-diaminoheptanedioate | mutant R451A, pH 8.5, 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate | relative activity wild-type: 14% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Ala-D-Ala | relative activity wild-type: 5% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Glu | relative activity wild-type: 13% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Lys | relative activity wild-type: 9% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + D-ornithine | relative activity wild-type: 8% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + DL-lanthionine | relative activity wild-type: 82% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + L-Ala | relative activity wild-type: 12% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + L-cystathionine | relative activity wild-type: 12% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + L-Lys | relative activity wild-type: 7% | Mycobacterium tuberculosis | ? | - |
? | |
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate | relative activity wild-type: 100% | Mycobacterium tuberculosis | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
GTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate | relative activity wild-type: 30% | Mycobacterium tuberculosis | GDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate | relative activity wild-type: 17% | Mycobacterium tuberculosis | UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurE | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | mutant N449D, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | |
0.06 | - |
ATP | mutant R451A, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | |
0.08 | - |
ATP | mutant K157A, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | |
0.1 | - |
ATP | mutant D392A, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | |
0.56 | - |
ATP | mutant E220A, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | |
1.11 | - |
ATP | N-terminal deletion mutant, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | |
1.3 | - |
ATP | wild-type, pH 8.5, 37°C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Mycobacterium tuberculosis |