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Literature summary for 6.3.2.13 extracted from

  • Mizuno, Y.; Ito, E.
    Purification and properties of uridine diphosphate N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase (1968), J. Biol. Chem., 243, 2665-2672.
    View publication on PubMed
Search for more literature for 6.3.2.13

Inhibitors

Inhibitors Comment Organism Structure
UDP-MurNAc-L-Ala-D-Glu above 0.4 mM Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 meso-2,6-diaminoheptanedioate
-
 Bacillus cereus
0.32
-
 UDP-N-MurNAc-L-Ala-D-Glu
-
 Bacillus cereus
0.34
-
 ATP
-
 Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ K+ or NH4+ required Bacillus cereus
Mg2+ Mg2+ or Mn2+ required Bacillus cereus
Mg2+ optimal concentration of Mg2+ is 10 mM Bacillus cereus
Mn2+ Mg2+ or Mn2+ required. With Mn2+ maximal activity is found at concentrations to half that of ATP Bacillus cereus
NH4+ K+ or NH4+ required Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.15
-
-
 Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate r Bacillus cereus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
-
 Bacillus cereus UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3 9
-
 Bacillus cereus