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Literature summary for 6.3.2.1 extracted from

  • von Delft, F.; Lewendon, A.; Dhanaraj, V.; Blundell, T.L.; Abell, C.; Smith, A.G.
    The crystal structure of E. coli pantothenate synthetase confirms it as a member of the cytidylyltransferase superfamily (2001), Structure, 9, 439-450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
native and selenomethionine labeled PS, hanging drop vapor diffusion, hanging drops are composed of equal volumes of PS and reservoir solutions consisting of 50 mM Tris-HCl, pH 8.0, 4%-6% polyethylene glycol 4000, crystals appeare at 19°C after 2-4 days, crystals diffract to 1.7 A Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
(R)-pantoate
-
Escherichia coli
0.1
-
ATP
-
Escherichia coli
0.15
-
beta-Alanine
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Escherichia coli
65000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Escherichia coli
-
AMP + diphosphate + (R)-pantothenate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant pantothenate synthetase Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Escherichia coli AMP + diphosphate + (R)-pantothenate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
Pantothenate synthetase
-
Escherichia coli
PS
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Escherichia coli