Literature summary for 6.3.1.9 extracted from

Wyllie, S.; Oza, S.L.; Patterson, S.; Spinks, D.; Thompson, S.; Fairlamb, A.H.
Dissecting the essentiality of the bifunctional trypanothione synthetase-amidase in Trypanosoma brucei using chemical and genetic methods (2009), Mol. Microbiol., 74, 529-540.

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Protein Variants

Protein Variants	Comment	Organism
C57A	functional synthetase devoid of amidase activity. In the presence of inducer, this line showed decreased growth in vitro and decreased virulence in vivo, indicating that the amidase function is not absolutely required for viability	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

General Information

General Information	Comment	Organism
physiological function	culture of a tetracyclinedependent conditional double knockout cDKO parasites without tetracycline induction results in loss of trypanothione and accumulation of glutathione, followed by growth inhibition and cell lysis after 6 days. In the absence of inducer, cDKO cells are unable to infect mice, confirming that the enzyme is essential for virulence in vivo as well as in vitro	Trypanosoma brucei

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Release 2023.1 (January 2023)